The church, which until the beginning of XIX century was an Orthodox cathedral, was visited by the Emperor Paul I. During the War of 1812, the church housed a French hospital. During the Second World War the church served as a refuge from the bombing for the residents of the city. Until the early 1990s the building was an archive and since 1991, services were resumed in the church.

On 6 April 1999, eight newly cast bells were consecrated and raised to the belfry. In January 2001, their number was supplemented by another on, the biggest.

The church keep revered icon of the Vilna Martyrs Anthony, John, and Eustathius with the particles of the relics.